p_nitrogen=ggplot(data=mean_flux ,aes(x=day, y=nitrogen,
fill=factor(Fertilizer.type) )) + theme_bw() +
geom_bar(width=pos,position=position_dodge(pos),stat="identity")+
geom_errorbar(aes(ymin=mean_flux$nitrogen-sd_flux$nitrogen,ymax=mean_flux$nitrogen+sd_flux$nitrogen),
width=pos/2,
position=position_dodge(pos))+
#geom_line(aes(color = Fertilizer.type), position = position_dodge(width = 1))+
xlab(labs$no) +
ylab(labs$nitrogenn)+
scale_fill_manual(values=c("red", "blue", "green","grey", "yellow"))+
#scale_fill_brewer(palette="BrBG") + # color adjustment
s.theme1+ # add x and y axis theme here
s.theme2+ # add-legend theme here
facet + # third factor
facect.t + # facet adjustment
scale_y_continuous(limits = c(0, 4.00), breaks = seq(0, 4.00, by = 0.5))+
scale_x_continuous(breaks=c(1,4,6,8,11,14,18,20,22,25,27,29,32,34,36,39,41,43,46,49,53,55,57,60,62,64,67))+
theme(legend.position = "top")+ # facet adjustment
labs(fill = "Fertilizer type") # correct legend title
p_nitrogen
ggsave("p_nitrogen-bar0001.png", width = 20, height =10)

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